permeabilization buffer (Thermo Fisher)

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Thermo Fisher permeabilization buffer
Permeabilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer+a/Triton+X-100/pm42332882-110-12-19
Average 99 stars, based on 1 article reviews

permeabilization buffer - by Bioz Stars, 2026-10

99/100 stars

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Incubation:

Article Title: Enrichment of Neural Crest Cells by Antibody Labeling and Flow Cytometry for Single-Cell Transcriptomics in a Lizard.
Article Snippet: .. The fixed cell solutions were resuspended in a permeabilization buffer (PB; 2% BSA, 100U/mL RNase‐inhibitor, 5 mM 1,4‐ Dithiothreitol, 0.1 M MOPS pH 7.5, 1 mM EGTA, 2 mM MgSO4, 125 mM NaCl and 0.1% Saponin), and incubated on ice for 5 min. Once permeabilized, the cells were stained with a mix of DAPI (1:1000; ThermoScientific 62248), which stains DNA, and a FITC‐conjugated HNK‐1 antibody (1:100; Anti‐Hu CD57 eBioscience 11‐057742) in MRDB (a buffer identical to PB but lacking saponin), followed by a washing step with the same buffer and passed through a 70 μm cell strainer. ..

Article Title: Magnetic resonance identification tags for ultra-flexible electrodes.
Article Snippet: Once deep anesthesia was confirmed, transcardial perfusion was performed with 1x PBS (pH 7.4, Gibco, Thermo Fisher Scientific, Cat. No. 10010056) followed by 4% paraformaldehyde (PFA) in PBS, freshly prepared from powder (Merck, Cat. No. 158127). .. For immunostaining, free-floating sections were first incubated in a permeabilization solution containing 0.3% Triton X-100 in PBS (pH 7.4, Gibco, Thermo Fisher Scientific, Cat. No. 10010056) for 30 minutes at room temperature with gentle shaking. ..

Article Title: Multiscale Hybrid Surface Topographies Orchestrate Immune Regulation, Antibacterial Defense, and Tissue Regeneration.
Article Snippet: The surface morphology of the wrinkled structures was further examined using scanning electron microscopy (SEM, Tescan Mira3). .. Immunostaining Assay for Macrophages: RAW 264.7 macrophages were seeded onto the test samples at a density of 2 × 104 cells and incubated for 24 h. After incubation, the cells were fixed and treated with a permeabilization/blocking buffer (PBS containing 5% normal goat serum and 0.3% Triton X-100) for 60 min. Once the buffer was removed, a cocktail of CD206 monoclonal antibody (MR5D3, rat/IgG2a, Invitrogen, 1:200) and iNOS monoclonal antibody (D6B6S, rabbit/IgG, Cell Signaling Technology, 1:200) in staining buffer (PBS with 1% BSA and 0.3% Triton X100) was added. ..

Article Title: Enrichment of Neural Crest Cells by Antibody Labeling and Flow Cytometry for Single‐Cell Transcriptomics in a Lizard
Article Snippet: .. The fixed cell solutions were resuspended in a permeabilization buffer (PB; 2% BSA, 100U/mL RNase‐inhibitor, 5 mM 1,4‐Dithiothreitol, 0.1 M MOPS pH 7.5, 1 mM EGTA, 2 mM MgSO4, 125 mM NaCl and 0.1% Saponin), and incubated on ice for 5 min. Once permeabilized, the cells were stained with a mix of DAPI (1:1000; ThermoScientific 62248), which stains DNA, and a FITC‐conjugated HNK‐1 antibody (1:100; Anti‐Hu CD57 eBioscience 11‐057742) in MRDB (a buffer identical to PB but lacking saponin), followed by a washing step with the same buffer and passed through a 70 μm cell strainer. ..

Article Title: Longitudinal in vivo imaging of dynamic immune-vascular responses to meningeal cerebrovascular injury.
Article Snippet: .. Afterwards, samples were further permeabilized with a 10- minute incubation in a permeabilization buffer (0.1% Triton X-100 and 1% sodium citrate (Thermo Fischer CAT #J63888.AK) in PBS). ..

Article Title: Longitudinal in vivo imaging of dynamic immune–vascular responses to meningeal cerebrovascular injury
Article Snippet: .. Afterwards, samples were further permeabilized with a 10-minute incubation in a permeabilization buffer (0.1% Triton X-100 and 1% sodium citrate (Thermo Fischer CAT #J63888.AK) in PBS). .. Sections were washed twice for 5 min with PBST and stained with TUNEL using kit instructions (TUNEL Assay Apoptosis Detection Kit, Biotium CAT #30074).

Article Title: Live longitudinal imaging of meningeal cerebrovascular injury and its sequelae in adult zebrafish
Article Snippet: .. Afterwards, samples were further permeabilized with a 10-minute incubation in a permeabilization buffer (0.1% Triton X-100 and 1% sodium citrate (Thermo Fischer CAT #J63888.AK) in PBS). .. Sections were washed twice for 5 minutes with PBST and stained with TUNEL using kit instructions (TUNEL Assay Apoptosis Detection Kit, Biotium CAT #30074).

Staining:

Immunostaining:

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other:

Article Title: Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasion
Article Snippet: For live cell tracking, the cell suspension was first incubated with 100ng/mL Hoechst stain (Thermo Scientific #H3570) for 45 minutes at 37°C, then centrifuged and resuspended in fresh media.

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